Anthracycline distribution and secondary alcohol metabolite formation in human myocardial strips
Human myocardial strips were incubated in plasma with 10 μM anthracyclines, as described under Materials and Methods. Values are means ± S.E. of six to 20 experiments and were analyzed by one-way analysis of variance followed by Bonferroni's test for multiple comparisons.
Anthracycline | Distribution | Secondary Alcohol Metabolite | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Membrane Fraction | Soluble Fraction | Membrane Fraction | Soluble Fraction | Plasma | Total | |||||
| μM | ||||||||||
| Doxorubicinone | 1.7 ± 0.4a | 1.1 ± 0.2a | 0.09 ± 0.01 | 0.074 ± 0.004 | 0.26 ± 0.06 | 0.43 ± 0.05c | ||||
| Doxorubicin | 4.2 ± 0.2b | 4.9 ± 0.6 | 0.049 ± 0.01 | 0.049 ± 0.01d | ||||||
| Epirubicin | 5.8 ± 0.6 | 4.2 ± 0.5 |
| 0.028 ± 0.003 |
| 0.028 ± 0.003 | ||||
↵ a p < 0.05 for doxorubicinone vs. doxorubicin or epirubicin in the membrane or soluble fraction.
↵ b p < 0.05 for doxorubicin vs. epirubicin in the membrane fraction.
↵ c p < 0.001 for doxorubicinolone vs. doxorubicinol or epirubicinol.
↵ d p > 0.05 for doxorubicinol vs. epirubicinol (p < 0.001 by unpaired Student's t test).