TABLE 2

Cellular localization of EYFP-hCAR in CAR-null mice

CAR^–/– mice were tail vein-injected with 10 μ g of EYFP-hCAR and treated 2 and 5 h later by intraperitoneal injection of vehicle (DMSO, CTL), 50 mg/kg RIF, 100 mg/kg PB, 10 mg/kg CITCO, 20 mg/kg CMZ, 25 mg/kg EFV, or 50 mg/kg NVP at volumes of 0.1 ml per 20 g body weight. EYFP-hCAR expression was detected in mouse liver sections by confocal laser scanning microscope. For each treatment, over 150 hCAR-expressing cells from two mice livers (M1 and M2) were counted and classified as having cytosolic (C), nuclear (N), or mixed (C + N) hCAR localization. Numbers in parentheses represent the percentages of cells exhibiting the specified localization relative to the total number of counted cells. M1 = mouse 1, M2 = mouse 2.