TABLE 1

Oligonucleotide sequences used for the gel shift analyses

The sequences of ER6/3A4 and DR4/2B6 contain the ER6 in the human CYP3A4 promoter (Lehmann et al., 1998) and the DR4 in the CYP2B6 promoter (Sueyoshi et al., 1999), respectively. The hexamer half-sites are indicated by capital letters.


Probe

Sequence

Position
ER6a/3A4 5′-tagaataTGAACTcaaaggAGGTCAgtcagt-3′ -279/-249
DR4b/2B6 5′-tcttcaGGGTCAggaaAGTACAgt-3′ -1734/-1711
-6698/2A6 5′-gcgggcAGATCAcctgAGGTCAggagttcgagacca-3′ -6704/-6669
-5476/2A6 5′-gggcGGATCActtaAGGTCAggag-3′ -5480/-5457
-4618/2A6
5′-agtgggcAGATCAcctgAGGTCAggagttt-3′
-4625/-4596
  • a ER6, everted repeat separated by six nucleotides.

  • b DR4, direct repeat separated by four nucleotides.