PT - JOURNAL ARTICLE AU - Koji Nobe AU - Akiko Fujii AU - Kiyomi Saito AU - Takaharu Negoro AU - Yoshio Ogawa AU - Yasuko Nakano AU - Terumasa Hashimoto AU - Kazuo Honda TI - Adiponectin Enhances Calcium Dependency of Mouse Bladder Contraction Mediated by Protein Kinase C<em>α</em> Expression AID - 10.1124/jpet.112.202028 DP - 2013 Apr 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 62--68 VI - 345 IP - 1 4099 - http://jpet.aspetjournals.org/content/345/1/62.short 4100 - http://jpet.aspetjournals.org/content/345/1/62.full SO - J Pharmacol Exp Ther2013 Apr 01; 345 AB - Adiponectin is an adipose tissue–secreted protein and is a multifunctional adipocytokine. However, the association of adiponectin with bladder contraction has not been investigated. In this study, the adiponectin-sense transgenic mouse (Adip-Sen mouse; age, 16–24 weeks; male) and age-matched controls (C57Bl mouse) were studied. The Adip-Sen mouse showed a significant increase in plasma adiponectin levels (56.2%; P &lt; 0.01), compared with those in the C57Bl mouse, without affecting other lipid parameters. Isometric force development in bladder smooth muscle tissues were detected using an organ-bath system. Although carbachol (CCh)–induced (0.1–100 µM) time- and dose-dependent contractions in Adip-Sen mouse bladder were slightly enhanced, compared with those in the C57Bl mouse during a low range (0.3–1.0 µM) of CCh, differences could not be detected with other CCh concentrations. However, the reduction in contraction under Ca2+-replaced conditions was significantly different between Adip-Sen and C57Bl mice (94.1 and 66.3% of normal contraction, respectively; n = 5). A parameter of Ca2+ sensitivity, the relation between intracellular Ca2+ concentration and contraction, was increased in the Adip-Sen mouse, compared with that in the C57B1 mouse. This Ca2+ dependency in the Adip-Sen mouse was reduced by a protein kinase C (PKC) inhibitor, but not by a Rho kinase inhibitor. Expression of the calcium-dependent isoform of PKC, PKCα, was increased in the Adip-Sen mouse bladder, and CCh-induced phosphorylation of PKCα was also enhanced, compared with those in the C57Bl mouse. In conclusion, adiponectin is associated with bladder smooth muscle contraction, which involves an increase in Ca2+ dependency of contraction mediated by PKCα expression.