PT  - JOURNAL ARTICLE
AU  - FRED E. D'AMOUR
AU  - R. G. GUSTAVSON
TI  - A BIOLOGIC ASSAY OF "INTERNATIONAL STANDARD" ESTRIN AND OF CERTAIN COMMERCIAL PREPARATIONS
DP  - 1936 Aug 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 472--481
VI  - 57
IP  - 4
4099  - http://jpet.aspetjournals.org/content/57/4/472.short
4100  - http://jpet.aspetjournals.org/content/57/4/472.full
SO  - J Pharmacol Exp Ther1936 Aug 01; 57
AB  - A thorough biologic assay was made of International Standard Estrin. It was found that the rat unit, i.e., the amount necessary to produce a positive reaction in 50 per cent of a large group of spayed rats, was as follows: Single injection in oil, 1.3 gamma; multiple injection in oil, 1.5 gamma; single injection, aqueous, 3.8 gamma; multiple injection, aqueous, 0.74 gamma; multiple injection, emulsion, 0.76 gamma. An assay of certain commercial estrogenic preparations indicated, for oil solutions, that the biologic activity, per International Unit stated by the label to be present, corresponded with the activity of the International Standard. For aqueous preparations, the biologic activity, per International Unit stated by the label to be present was only one-sixth to one-seventh as great as the biologic activity per International Unit of the International Standard material.