RT Journal Article
SR Electronic
T1 THE PREPARATION OF PROLACTIN
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 365
OP 371
VO 55
IS 3
A1 ROBERT W. BATES
A1 OSCAR RIDDLE
YR 1935
UL http://jpet.aspetjournals.org/content/55/3/365.abstract
AB A procedure for extraction and proximate purification of prolactin is fully described. The tissue is first extracted with 60 to 70 per cent aqueous ethanol at pH 9 to 10, and this followed by complete precipitation of the active principles at a higher ethanol concentration and at pH 6.0. The prolactin is next separated from the bulk of follicle-stimulating and thyreotropic hormones by taking advantage of the insolubility of prolactin between pH 3 to 4 in the presence of sulphates. The hormone is further purified through use of its solubility in aqueous ethanol. About 70 per cent of the prolactin present in the original tissue is thus obtained in one fraction uncontaminated by F. S. H. and thyreotropic hormone. At pH 8.0 prolactin can be heated to boiling for one hour or to 60°C. for five hours without great loss of potency.