RT Journal Article
SR Electronic
T1 Assessing Antibody Pharmacokinetics in Mice with In Vivo Imaging
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 350
OP 358
DO 10.1124/jpet.110.172916
VO 337
IS 2
A1 Hoppin, Jack
A1 Orcutt, Kelly Davis
A1 Hesterman, Jacob Y.
A1 Silva, Matthew D.
A1 Cheng, Dengfeng
A1 Lackas, Christian
A1 Rusckowski, Mary
YR 2011
UL http://jpet.aspetjournals.org/content/337/2/350.abstract
AB Recent advances in small-animal molecular imaging instrumentation combined with well characterized antibody-labeling chemistry have enabled detailed in vivo measurements of antibody distribution in mouse models. This article reviews the strengths and limitations of in vivo antibody imaging methods with a focus on positron emission tomography and single-photon emission computed tomography and a brief discussion of the role of optical imaging in this application. A description of the basic principles behind the imaging techniques is provided along with a discussion of radiolabeling methods relevant to antibodies. Practical considerations of study design and execution are presented through a discussion of sensitivity and resolution tradeoffs for these techniques as defined by modality, signaling probe (isotope or fluorophore) selection, labeling method, and radiation dosimetry. Images and analysis results from a case study are presented with a discussion of output data content and relevant informatics gained with this approach to studying antibody pharmacokinetics.