TY - JOUR T1 - Assessing Antibody Pharmacokinetics in Mice with In Vivo Imaging JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 350 LP - 358 DO - 10.1124/jpet.110.172916 VL - 337 IS - 2 AU - Jack Hoppin AU - Kelly Davis Orcutt AU - Jacob Y. Hesterman AU - Matthew D. Silva AU - Dengfeng Cheng AU - Christian Lackas AU - Mary Rusckowski Y1 - 2011/05/01 UR - http://jpet.aspetjournals.org/content/337/2/350.abstract N2 - Recent advances in small-animal molecular imaging instrumentation combined with well characterized antibody-labeling chemistry have enabled detailed in vivo measurements of antibody distribution in mouse models. This article reviews the strengths and limitations of in vivo antibody imaging methods with a focus on positron emission tomography and single-photon emission computed tomography and a brief discussion of the role of optical imaging in this application. A description of the basic principles behind the imaging techniques is provided along with a discussion of radiolabeling methods relevant to antibodies. Practical considerations of study design and execution are presented through a discussion of sensitivity and resolution tradeoffs for these techniques as defined by modality, signaling probe (isotope or fluorophore) selection, labeling method, and radiation dosimetry. Images and analysis results from a case study are presented with a discussion of output data content and relevant informatics gained with this approach to studying antibody pharmacokinetics. ER -