PT  - JOURNAL ARTICLE
AU  - Jack Hoppin
AU  - Kelly Davis Orcutt
AU  - Jacob Y. Hesterman
AU  - Matthew D. Silva
AU  - Dengfeng Cheng
AU  - Christian Lackas
AU  - Mary Rusckowski
TI  - Assessing Antibody Pharmacokinetics in Mice with In Vivo Imaging
AID  - 10.1124/jpet.110.172916
DP  - 2011 May 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 350--358
VI  - 337
IP  - 2
4099  - http://jpet.aspetjournals.org/content/337/2/350.short
4100  - http://jpet.aspetjournals.org/content/337/2/350.full
SO  - J Pharmacol Exp Ther2011 May 01; 337
AB  - Recent advances in small-animal molecular imaging instrumentation combined with well characterized antibody-labeling chemistry have enabled detailed in vivo measurements of antibody distribution in mouse models. This article reviews the strengths and limitations of in vivo antibody imaging methods with a focus on positron emission tomography and single-photon emission computed tomography and a brief discussion of the role of optical imaging in this application. A description of the basic principles behind the imaging techniques is provided along with a discussion of radiolabeling methods relevant to antibodies. Practical considerations of study design and execution are presented through a discussion of sensitivity and resolution tradeoffs for these techniques as defined by modality, signaling probe (isotope or fluorophore) selection, labeling method, and radiation dosimetry. Images and analysis results from a case study are presented with a discussion of output data content and relevant informatics gained with this approach to studying antibody pharmacokinetics.