PT - JOURNAL ARTICLE AU - Raffi Maghdessian AU - François Côté AU - Thérèse Rouleau AU - Ali Ben Djoudi Ouadda AU - Émile Levy AU - Jean-Claude Lavoie TI - Ascorbylperoxide Contaminating Parenteral Nutrition Perturbs the Lipid Metabolism in Newborn Guinea Pig AID - 10.1124/jpet.110.166223 DP - 2010 Jul 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 278--284 VI - 334 IP - 1 4099 - http://jpet.aspetjournals.org/content/334/1/278.short 4100 - http://jpet.aspetjournals.org/content/334/1/278.full SO - J Pharmacol Exp Ther2010 Jul 01; 334 AB - The light exposure of parenteral nutritive solutions generates peroxides such as H2O2 and ascorbylperoxide [2,3-diketo-4-hydoxyperoxyl-5,6-dihydroxyhexanoic acid]. This absence of photoprotection is associated with higher plasma triacylglycerol (TG) concentration in premature infants and oxidative stress and H2O2-independent hepatic steatosis in animals. We hypothesized that ascorbylperoxide is the active agent leading to high TG. The aim was to investigate the role of ascorbylperoxide in glucose and lipid metabolism in an animal model of neonatal parenteral nutrition. Three-day-old guinea pigs received through a catheter in the jugular solutions containing dextrose plus 0, 90, 225, or 450 μM ascorbylperoxide. After 4 days, blood and liver were sampled and treated for determinations of TG, cholesterol, markers of oxidative stress (redox potential of glutathione and F2α-isoprostane), and activities and protein levels of acetyl-CoA carboxylase (ACC), glucokinase, and phosphofructokinase (PFK). Ascorbylperoxide concentration was measured in urine on the last day. Data were compared by analysis of variance (p < 0.05). Plasma TG and cholesterol and hepatic PFK activity increased (200% of control), whereas ACC activity decreased (66% of control) in the function of the amount of ascorbylperoxide infused. Both markers of oxidative stress were higher in animals receiving the highest amounts of ascorbylperoxide. The logarithmic relations between urinary ascorbylperoxide and plasma TG (r2 = 0.69) and hepatic PFK activity (r2 = 0.26) were positive, whereas they were negative with ACC activity (r2 = 0.50). In conclusion, ascorbylperoxide contaminating parenteral nutrition stimulates glycolysis, allowing higher availability of substrates for lipid synthesis. The logarithmic relation between urinary ascorbylperoxide and plasma TG suggests a very low efficient concentration. Copyright © 2010 by The American Society for Pharmacology and Experimental Therapeutics