PT - JOURNAL ARTICLE AU - Dong-Mei Chen AU - Liang Xiao AU - Xin Cai AU - Rong Zeng AU - Xing-Zu Zhu TI - Involvement of Multitargets in Paeoniflorin-Induced Preconditioning AID - 10.1124/jpet.106.104380 DP - 2006 Oct 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 165--180 VI - 319 IP - 1 4099 - http://jpet.aspetjournals.org/content/319/1/165.short 4100 - http://jpet.aspetjournals.org/content/319/1/165.full SO - J Pharmacol Exp Ther2006 Oct 01; 319 AB - Paeoniflorin (PF) is the principal component of Paeoniae radix prescribed in traditional Chinese medicine. The delayed neuroprotection induced by PF preconditioning and its underlying mechanisms were investigated in rat middle cerebral artery occlusion (MCAO) and reperfusion model. At a dosage of 20 or 40 mg/kg, PF preconditioning 48 h before MCAO followed by 24-h reperfusion significantly reduced the mortality and infarct volume and reversed the neurological deficits caused by ischemia. Likewise, the ameliorative effects on mortality, infarct size, and neurological impairment induced by MCAO emerged as well when PF was administered 24 h, 48 h, or 5 days before MCAO at the dose of 20 mg/kg. Furthermore, comparative proteomics analysis was adopted to identify the differentially expressed proteins induced by PF preconditioning itself. The relative levels of 42 proteins were altered after PF preconditioning, among which 20 were elevated and 22 reduced. In summary, A1 receptor-regulator of G protein signaling-KATP signaling, arachidonic acid cascade, nitric oxide system, markers of neuronal damage, mitochondrial damage-related molecules, and the mitogen-activated protein kinase and nuclear factor-κB pathway are associated with the mechanisms of PF preconditioning. The American Society for Pharmacology and Experimental Therapeutics