TY - JOUR T1 - A Novel Effect for Annexin 1-Derived Peptide Ac2-26: Reduction of Allergic Inflammation in the Rat JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 1416 LP - 1422 DO - 10.1124/jpet.104.080473 VL - 313 IS - 3 AU - Christianne Bandeira-Melo AU - André Gustavo C. Bonavita AU - Bruno L. Diaz AU - Patricia M. R. e Silva AU - Vinícius F. Carvalho AU - Peter J. Jose AU - Roderick J. Flower AU - Mauro Perretti AU - Marco A. Martins Y1 - 2005/06/01 UR - http://jpet.aspetjournals.org/content/313/3/1416.abstract N2 - Previous investigations have provided evidence that the N-terminal peptide of annexin 1 (peptide Ac2-26) has the capacity of reproducing the anti-inflammatory actions of the full-length protein in many systems. In the current study, we report the effectiveness of the peptide Ac2-26 as an antiallergic tool in a model of rat pleurisy and provide indication for some of the mechanisms involved. In rats inflamed by injection of ovalbumin into the pleural cavity 14 days postsensitization, peptide Ac2-26 (50–200 μg/cavity) inhibited mast cell degranulation, plasma protein leakage, and the accumulation of both neutrophils and eosinophils. Treatment with either peptide Ac2-26 (200 μg/cavity) or dexamethasone (1 mg/kg i.p.) inhibited ovalbumin-induced eotaxin release in the pleural effluents. In vitro, peptide Ac2-26 inhibited ovalbumin-evoked histamine release from subcutaneous tissue fragments obtained from sensitized rats (33–66 μM) and interleukin-13-evoked eotaxin generation from cultured rat mesothelial cells (16–33 μM) but not eosinophil chemotaxis. This work demonstrates that the annexin 1 mimetic peptide Ac2-26 prevents allergen-evoked eosinophilic inflammatory response in rats. Combined analysis of the in vivo and in vitro experiments presented herein suggests that the blockade of secretion of pivotal mediators for the allergic response, such as histamine and eotaxin, could be responsible for the inhibitory actions displayed by peptide Ac2-26. The American Society for Pharmacology and Experimental Therapeutics ER -