%0 Journal Article %A Régis Steinberg %A Richard Alonso %A Liliane Rouquier %A Christophe Desvignes %A Jean-Claude Michaud %A Annie Cudennec %A Mireille Jung %A Jacques Simiand %A Guy Griebel %A Xavier Emonds-Alt %A Gérard Le Fur %A Philippe Soubrié %T SSR240600 [(R)-2-(1-{2-[4-{2-[3,5-Bis(trifluoromethyl)phenyl]acetyl}-2-(3,4-dichlorophenyl)-2-morpholinyl]ethyl}-4-piperidinyl)-2-methylpropanamide], a Centrally Active Nonpeptide Antagonist of the Tachykinin Neurokinin 1 Receptor: II. Neurochemical and Behavioral Characterization %D 2002 %R 10.1124/jpet.102.040279 %J Journal of Pharmacology and Experimental Therapeutics %P 1180-1188 %V 303 %N 3 %X SSR240600 [(R)-2-(1-{2-[4-{2-[3,5-bis(trifluoromethyl)phenyl]acetyl}-2-(3,4-dichlorophenyl)-2-morpholinyl]ethyl}-4-piperidinyl)-2-methylpropanamide], a new nonpeptide tachykinin neurokinin 1 (NK1) receptor antagonist, was evaluated against the neurochemical, electrophysiological, and behavioral effects provoked by direct activation of brain tachykinin NK1 receptors or by stress in guinea pigs. SSR240600 (0.1–10 mg/kg i.p. or p.o.) antagonized the excitatory effect of i.c.v. infusion of [Sar9,Met(O2)11]substance P (SP) on the release of acetylcholine in the striatum of anesthetized and awake guinea pigs. This antagonistic action was still observed after repeated administration of SSR240600 (5 days, 10 mg/kg p.o., once a day). SSR240600 (10 mg/kg i.p.) inhibited the phosphorylation of the cAMP response element-binding protein in various brain regions induced by i.c.v. administration of [Sar9,Met(O2)11]SP. In slice preparations, neuronal firing of the locus coeruleus (LC) neurons elicited by the application of [Sar9,Met(O2)11]SP was suppressed by SSR240600 at 100 nM. Norepinephrine release in the prefrontal cortex, elicited either by an intra-LC application of [Sar9,Met(O2)11]SP or by an i.c.v administration of corticotropin-releasing factor, was reduced by SSR240600 (0.3–1 mg/kg and 1–10 mg/kg i.p., respectively). SSR240600 (1–10 mg/kg i.p.) inhibited vocalizations induced in adult guinea pigs by an i.c.v. administration of the NK1 receptor agonist, GR73632 [d-Ala-[l-Pro9,Me-Leu8]substance P(7-11)]. Furthermore, SSR240600 (1–10 mg/kg i.p.) inhibited distress vocalizations produced in guinea pig pups by maternal separation. SSR240600 also reduced maternal separation-induced increase in the number of neurons displaying NK1 receptor internalization in the amygdala. Finally, SSR240600 counteracted the increase in body temperature induced by isolation stress. In conclusion, SSR240600 is able to antagonize various NK1receptor-mediated as well as stress-mediated effects in the guinea pig. %U https://jpet.aspetjournals.org/content/jpet/303/3/1180.full.pdf