TY - JOUR T1 - Long-Lasting Facilitation of 4-Amino-<em>n</em>-[2,3-<sup>3</sup>H]butyric Acid ([<sup>3</sup>H]GABA) Release from Rat Hippocampal Slices by Nicotinic Receptor Activation JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 453 LP - 462 VL - 295 IS - 2 AU - Attila Köfalvi AU - Beáta Sperlágh AU - Tibor Zelles AU - E. Sylvester Vizi Y1 - 2000/11/01 UR - http://jpet.aspetjournals.org/content/295/2/453.abstract N2 - In this study we explored the effect of the stimulation of nicotinic acetylcholine receptors located on interneurons by measuring 4-amino-n-[2,3-3H]butyric acid ([3H]GABA) release and monitoring [Ca2+]i in superfused hippocampal slices. In the presence of 6-cyano-7-nitroquinoxaline-2,3-dione, (±)-2-amino-5-phosphonopentanoic acid, and atropine, i.e., under the blockade of N-methyl-d-aspartate and non-N-methyl-d-aspartate glutamate and muscarinic receptors, nicotine did not alter the spontaneous outflow of [3H]GABA, but significantly increased the stimulation-evoked [3H]GABA efflux. This effect of nicotine depended on the time interval between nicotine treatment and electrical stimulus, the concentration of nicotine (1–100 μM), and the parameters of electrical depolarization. Acetylcholine (0.03–3 mM), and the α7 subtype-selective agonist choline (0.1–10 mM), also potentiated stimulus-evoked release of [3H]GABA, whereas 1,1-dimethyl-4-phenilpiperazinium iodide failed to increase the tritium outflow significantly. The effect of nicotine treatment was prevented by tetrodotoxin (1 μM) and by the nicotinic acetylcholine receptor antagonist mecamylamine (10 μM), and the α7 subtype-selective antagonists α-bungarotoxin (100 nM) and methyllycaconitine (10 nM), whereas dihidro-β-erythroidine (20 nM) was without effect. Perfusion of 100 μM nicotine caused a [Ca2+]itransient in about one-third of the tested interneurons; however, the response to subsequent electrical stimulation remained unchanged. Inhibition of the GABA transporter system by nipecotic acid (1 mM) or by decreasing the bath temperature to 12°C abolished completely the effect of nicotine to potentiate the stimulation-evoked release of GABA. These findings indicate that the activation of α7-type nicotinic receptors of hippocampal interneurons results in a long-lasting ability of these cells to respond to depolarization with an increased release of GABA mediated by the transporter system. The American Society for Pharmacology and Experimental Therapeutics ER -