TY - JOUR T1 - Effects of Chronic Ethanol Treatment on γ-Aminobutyric Acid<sub>A</sub> and Glycine Receptors in Mouse Glycinergic Spinal Neurons JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 423 LP - 429 VL - 295 IS - 1 AU - Brigitte van Zundert AU - Felipe A. Albarran AU - Luis G. Aguayo Y1 - 2000/10/01 UR - http://jpet.aspetjournals.org/content/295/1/423.abstract N2 - Five-day-old cultures of mouse glycinergic spinal interneurons were chronically treated with 100 mM ethanol and the glycine and γ-aminobutyric acid (GABA)A receptors were assayed using whole-cell recordings and fluorescence-imaging techniques. Control neurons displayed a glycine50 of 19 ± 0.6 μM and a Hill coefficient of 3.1 ± 0.3. Chronic ethanol treatment did not significantly change these parameters. The maximal responses were 310 ± 80 pA/pF in control and 440 ± 19 pA/pF in treated cells, and the fluorescence intensity associated to a monoclonal glycine receptor antibody was unchanged. Strychnine inhibited the glycine current with smaller potency (29%) in treated neurons, thus the IC50 increased from 14 ± 2 nM in control to 18 ± 6 nM in treated neurons. Zn2+ (10 μM) potentiated the glycine current by 43 ± 33% in control, but only by 18 ± 13% in treated neurons. Interestingly, no change on the inhibition produced by a high concentration of Zn2+ was found in treated neurons. The inhibitory effect of picrotoxin on the glycine receptor, associated to a homomeric receptor, was eliminated with chronic ethanol, suggesting a faster switch to β-subunit-containing receptors. Unlike glycine receptors, the sensitivity of GABAA receptors to GABA, pentobarbital, diazepam, and Zn2+, as well as the fluorescence intensity associated to a high-affinity benzodiazepine analog was unchanged by chronic ethanol. In conclusion, we found that glycine receptors in spinal interneurons were altered by chronic ethanol treatment and this may reflect the expression of different subunits in control and treated neurons. GABAA receptors were resistant to the treatment. The American Society for Pharmacology and Experimental Therapeutics ER -