TY - JOUR T1 - Alterations in G-Proteins and β-Adrenergic Responsive Adenylyl Cyclase in Rat Urinary Bladder during Aging JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 969 LP - 974 VL - 294 IS - 3 AU - Ithaar H. Derweesh AU - Marcia A. Wheeler AU - Robert M. Weiss Y1 - 2000/09/01 UR - http://jpet.aspetjournals.org/content/294/3/969.abstract N2 - Decreased response of bladder to β-adrenergic stimulation with aging is related to decreased adenylyl cyclase activity and possibly to changes in guanine nucleotide regulatory protein (G-protein) content or function. G-protein content was quantified by Western blot analysis using antibodies to Gsα, Goα, and Giα in 21-day-old (weanling), 90-day-old (young adult), 6-month-old (adult), and 24-month-old (old) rat bladders. Gi/Go function in bladders with aging was measured by ADP-ribosylation with pertussis toxin. Content of Gsα, Goα, and Giα was lower in 90-day-old bladder than in 21-day-old bladder. Gsα content was similar in the 21-day-, 6-month-, and 24-month-old bladders. Giα content as well as pertussis toxin-catalyzed ADP-ribosylation was higher in 24-month-old bladders than in 21- and 90-day-old bladders. Pertussis toxin-catalyzed ADP-ribosylation of bladder membranes and treatment of bladder with protein kinase A inhibitors reversed the age-dependent decline in isoproterenol stimulation of adenylyl cyclase. Decreases in β-adrenergic-induced relaxation response with age in rat bladder are due in part to increases in the content and functional activity of pertussis toxin-sensitive G-protein. The American Society for Pharmacology and Experimental Therapeutics ER -