PT  - JOURNAL ARTICLE
AU  - Case, Jennifer
AU  - Davison, Cathy A.
TI  - Estrogen Alters Relative Contributions of Nitric Oxide and Cyclooxygenase Products to Endothelium-Dependent Vasodilation
DP  - 1999 Nov 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 524--530
VI  - 291
IP  - 2
4099  - http://jpet.aspetjournals.org/content/291/2/524.short
4100  - http://jpet.aspetjournals.org/content/291/2/524.full
SO  - J Pharmacol Exp Ther1999 Nov 01; 291
AB  - The purpose of this study was to determine the effects of in vivo estrogen manipulations on mechanisms of endothelium-dependent vasodilation. Ovary-intact, ovariectomized (OVX), or OVX with estrogen replacement (OVX + E2) female Sprague-Dawley rats were studied (n = 8). Mesenteric arteries (∼300 μm) were isolated, cannulated, and pressurized to 60 mm Hg in an arteriograph containing bicarbonate buffer and vessel diameter was monitored. Concentration-response curves to the endothelium-dependent histamine H1 agonist 2-thiazolylethylamine (2-TEA; 1 nM–100 μM) and to acetylcholine (1 nM–10 μM) were performed in preconstricted arteries. The effect ofNω-nitro-l-arginine (LNA; 100 μM) or LNA + indomethacin (INDO) (10 μM) on agonist-induced vasodilation was determined. There was no difference between treatment groups in the sensitivity of mesenteric arteries to 2-TEA or acetylcholine. LNA produced a significant decrease in sensitivity to 2-TEA in arteries from ovary-intact and OVX + E2 rats but not in those from OVX rats. The addition of INDO produced a small additional decrease in sensitivity to 2-TEA in arteries from ovary-intact rats, a significant decrease in OVX, and no shift in OVX + E2. LNA + INDO produced a similar degree of inhibition of the 2-TEA response in the three treatment groups. In contrast, when acetylcholine was used, the decrease in sensitivity produced by LNA or LNA + INDO was similar in the three rat groups. We conclude that estrogen increases the nitric oxide component of endothelium-dependent dilation and decreases the cyclooxygenase component. These effects of estrogen appear to be agonist-specific. Our findings suggest that estrogen modulates cross talk between the nitric oxide synthase and cyclooxygenase pathways of vasodilation. The American Society for Pharmacology and Experimental Therapeutics