RT Journal Article SR Electronic T1 Neuroprotection (Focal Ischemia) and Neurotoxicity (Electroencephalographic) Studies in Rats with AHN649, a 3-Amino Analog of Dextromethorphan and Low-Affinity N-Methyl-d-Aspartate Antagonist JF Journal of Pharmacology and Experimental Therapeutics JO J Pharmacol Exp Ther FD American Society for Pharmacology and Experimental Therapeutics SP 399 OP 408 VO 291 IS 1 A1 Frank C. Tortella A1 Paul Britton A1 Anthony Williams A1 Xi Chung May Lu A1 Amy H. Newman YR 1999 UL http://jpet.aspetjournals.org/content/291/1/399.abstract AB AHN649, an analog of dextromethorphan (DM) and a relatively selective low-affinity N-methyl-d-aspartate antagonist, was evaluated for neuroprotective effects using the rat intraluminal filament model of temporary middle cerebral artery occlusion. Rats were subjected to 2 h of focal ischemia followed by 72 h of reperfusion. In vehicle-treated rats, middle cerebral artery occlusion resulted in neurological deficits and severe infarction measuring 232 ± 25 mm3, representing approximately 25% contralateral hemispheric infarction. Post-treatment with AHN649 (0.156–20 mg/kg i.v.) or DM (0.156–10 mg/kg i.v.) significantly reduced cortical infarct volume by 40 to 60% compared with vehicle-control treatments. AHN649 neuroprotection was linear and dose dependent (ED50 = 0.80 mg/kg), whereas DM neuroprotection (ED50 = 1.25 mg/kg) was nonlinear and less effective at the higher doses (2.5–10 mg/kg). Although impaired neurological function scores improved in all groups by 24 to 72 h, the most dramatic improvement was associated with AHN649 treatments. In a rat electroencephalographic model of brain function, separate neurotoxicity experiments revealed that acute i.v. doses of DM caused seizures (ED50 = 19 mg/kg) and death (LD50 = 27 mg/kg). In contrast, AHN649 failed to induce seizure activity at doses up to 100 mg/kg (LD50= 79 mg/kg). Collectively, AHN649 is described as a potent, efficacious neuroprotective agent devoid of serious central nervous system neurotoxicity and possessing potential therapeutic value as antistroke treatment. Furthermore, the feasibility of targeting low-affinityN-methyl-d-aspartate-site ligands as postinjury therapy for ischemic brain injury has been confirmed. The American Society for Pharmacology and Experimental Therapeutics