TY - JOUR T1 - Nitric Oxide Is the Predominant Mediator for Neurogenic Vasodilation in Porcine Pial Veins JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 398 LP - 404 VL - 289 IS - 1 AU - Takaaki Ishine AU - Jun-Ge Yu AU - Yujiro Asada AU - Tony Jer-Fu Lee Y1 - 1999/04/01 UR - http://jpet.aspetjournals.org/content/289/1/398.abstract N2 - The innervation pattern and the vasomotor response of the potential transmitters in the porcine pial veins were investigated morphologically and pharmacologically. The porcine pial veins were more densely innervated by vasoactive intestinal polypeptide (VIP)- and neuropeptide Y-immunoreactive (I) fibers than were calcitonin gene-related peptide (CGRP)-I, choline acetyltransferase-I, Substance P (SP)-I, and NADPH diaphorase fibers. Serotonin (5-HT)-I fibers, which were not detected in normal control pial veins, were observed in isolated pial veins after incubation with 5-HT (1 μM). 5-HT-I fibers, however, were not observed when incubation with 5-HT was performed in the presence of guanethidine (1 μM), suggesting that 5-HT was taken up into the sympathetic nerves. In vitro tissue bath studies demonstrated that porcine pial veins in the presence of active muscle tone relaxed on applications of exogenous 5-HT, CGRP, SP, VIP, and sodium nitroprusside, whereas exogenous norepinephrine and neuropeptide Y induced only constrictions. Transmural nerve stimulation (TNS) did not elicit any response in pial veins in the absence of active muscle tone. However, in the presence of active muscle tone, pial veins relaxed exclusively on TNS. This tetrodotoxin-sensitive relaxation was not affected by receptor antagonists for VIP, CGRP, 5-HT, or SP but was blocked by l-glutamine (1 mM) and abolished byNω-nitro-l-arginine (10 μM) and Nω-nitro-l-arginine methyl ester (10 μM). The inhibition by l-glutamine,Nω-nitro-l-arginine, andNω-nitro-l-arginine methyl ester was reversed by l-arginine andl-citrulline but not by their d-enantiomers. These results demonstrate that the vasomotor effect of all potential transmitters except 5-HT in the pial veins examined resembles that in cerebral arteries. Although porcine pial veins receive vasodilator and constrictor nerves, a lack of constriction on TNS suggests that the dilator nerves that release nitric oxide may play a predominant role in regulating porcine pial venous tone. The American Society for Pharmacology and Experimental Therapeutics ER -