PT  - JOURNAL ARTICLE
AU  - Lockhart, Leslie K.
AU  - McNicol, Archibald
TI  - The Phospholipase C Inhibitor U73122 Inhibits Phorbol Ester-Induced Platelet Activation
DP  - 1999 May 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 721--728
VI  - 289
IP  - 2
4099  - http://jpet.aspetjournals.org/content/289/2/721.short
4100  - http://jpet.aspetjournals.org/content/289/2/721.full
SO  - J Pharmacol Exp Ther1999 May 01; 289
AB  - Activation of phospholipase C (PLC) is a central component of the signal transduction process in numerous cells, including platelets. U73122 has been widely used as a selective PLC inhibitor. In the present study, the effects of U73122 on platelet function have been further examined. Platelets were stimulated with collagen (via PLC-γ), the stable thromboxane mimetic U46619 (via PLC-β), or phorbol myristate acetate (PMA) via protein kinase C (PKC). Consistent with inhibition of PLC, U73122 inhibited platelet aggregation and [3H]-serotonin release in response to collagen and U46619 in a concentration-dependent manner. Similarly, U73122 blocked collagen-induced release of thromboxane A2. U73122 also inhibited U46619-induced [32P]phosphatidic acid production and phosphorylation of the major PKC substrate, pleckstrin. U73122 had no effect on PMA-induced pleckstrin phosphorylation, [3H]-serotonin release, or intracellular vacuole formation. However, U73122 did inhibit PMA-induced platelet aggregation and fibrinogen binding. Overall, these results suggest that U73122, in addition to its inhibition of PLC, also affects PKC-independent events that interfere with platelet aggregation. The American Society for Pharmacology and Experimental Therapeutics