RT Journal Article
SR Electronic
T1 Efflux of Intracellular α-Ketoglutarate Via p-Aminohippurate/Dicarboxylate Exchange in OK Kidney Epithelial Cells
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 422
OP 427
VO 285
IS 2
A1 Junya Nagai
A1 Ikuko Yano
A1 Yukiya Hashimoto
A1 Mikihisa Takano
A1 Ken-Ichi Inui
YR 1998
UL http://jpet.aspetjournals.org/content/285/2/422.abstract
AB The involvement of intracellular α-ketoglutarate (α-KG) in p-aminohippurate (PAH) transport was investigated in OK kidney epithelial cells. Efflux of intracellular α-KG from the OK cells to the basolateral side was increased by applying PAH to the basolateral side of the cells. In contrast, the intracellular α-KG concentration was not influenced by the addition of PAH. The α-KG efflux across the basolateral membrane induced by PAH was higher than that across the apical membrane. Probenecid inhibited the PAH-dependent α-KG efflux. The α-KG efflux to the basolateral side was saturable with increasing concentration of PAH in the basolateral medium. Antimycin A, a metabolic inhibitor, inhibited [14C]PAH uptake across the basolateral membrane of OK cells in a dose-dependent manner. In addition, both the α-KG efflux induced by PAH and the intracellular α-KG concentration were decreased by antimycin A dose-dependently. These results directly show that α-KG generated by intracellular metabolism is effluxed via PAH/dicarboxylate exchange in the basolateral membrane of OK cells. The American Society for Pharmacology and Experimental Therapeutics