PT  - JOURNAL ARTICLE
AU  - Isabelle Gorenne
AU  - Carlos Labat
AU  - Jean-Pierre Gascard
AU  - Xavier Norel
AU  - Nabila Nashashibi
AU  - Charles Brink
TI  - Leukotriene D<sub>4</sub> Contractions in Human Airways are Blocked by SK&F 96365, an Inhibitor of Receptor-Mediated Calcium Entry
DP  - 1998 Feb 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 549--552
VI  - 284
IP  - 2
4099  - http://jpet.aspetjournals.org/content/284/2/549.short
4100  - http://jpet.aspetjournals.org/content/284/2/549.full
SO  - J Pharmacol Exp Ther1998 Feb 01; 284
AB  - In human bronchial muscle preparations, nifedipine (3 μM) significantly inhibited the histamine, ACh and KCl contractions. However, the dihydropyridine did not modify the contractile responses induced by either leukotriene D4 (LTD4) or anti-human IgE (a-IgE). In human airways, SK&F 96365 (30 μM and 100 μM) markedly reduced the KCl and, at the higher concentration, LTD4 maximal contractions. In addition, when preparations were treated with nifedipine (3 μM), SK&F 96365 (100 μM) significantly blocked responses to both LTD4 and a-IgE. The calcium chelating agent ethylene glycol-bis (β-amino-ethyl ether) N,N,N′,N′-tetraacetic acid (4 mM) also inhibited the a-IgE-induced contractions. These data demonstrate that the nifedipine-resistant component of the LTD4 and a-IgE contractions was inhibited by SK&F 96365 and suggest that the cysteinyl-leukotriene receptor in human airways may be intimately linked with a receptor-operated calcium-entry mechanism. The American Society for Pharmacology and Experimental Therapeutics