PT - JOURNAL ARTICLE AU - John Q. Wang AU - Jacqueline F. McGinty TI - The Full D<sub>1</sub> Dopamine Receptor Agonist SKF-82958 Induces Neuropeptide mRNA in the Normosensitive Striatum of Rats: Regulation of D<sub>1</sub>/D<sub>2</sub> Interactions by Muscarinic Receptors DP - 1997 May 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 972--982 VI - 281 IP - 2 4099 - http://jpet.aspetjournals.org/content/281/2/972.short 4100 - http://jpet.aspetjournals.org/content/281/2/972.full SO - J Pharmacol Exp Ther1997 May 01; 281 AB - Neuropeptide and immediate early gene expression in striatonigral neurons of the normosensitive striatum is induced by mixed D1/D2 receptor agonists and indirect dopamine agonists, such as cocaine and amphetamine. Both D1 and D2 receptor antagonists block these events. In contrast, the partial D1 agonist, SKF-38393, does not evoke striatonigral gene expression in intact rats. These findings have contributed to the idea that both D1 and D2receptors must be stimulated to evoke gene expression in striatonigral neurons. How these “D1/D2 interactions” are accomplished is unclear in light of the controversy over whether striatonigral neurons express both D1 and D2receptors. This study addresses these issues by demonstrating that in intact rats 1) a full D1 receptor agonist, SKF-82958, induced behavioral activity and preprodynorphin (PPD) and substance P (SP) gene expression in medium spiny neurons in the dorsal, and especially, in the ventral striatum, 2) either a D1antagonist, SCH-23390, or a D2 antagonist, eticlopride, blocked these effects, 3) the muscarinic antagonist, scopolamine, augmented PPD and SP mRNA expression induced by SKF-82958 and prevented the ability of eticlopride to block SKF-82958-induced PPD and SP mRNAs and 4) the SKF-82958-induced increase in preproenkephalin mRNA in striatopallidal neurons was blocked by SCH-23390 or scopolamine but not by eticlopride. These data indicate that endogenous acetylcholine attenuates D1 receptor-stimulated PPD/SP gene expression in medium spiny neurons, mediates D1 receptor-stimulated preproenkephalin gene expression in striatopallidal neurons and contributes to D2 receptor involvement in D1-stimulated PPD/SP gene expression. The American Society for Pharmacology and Experimental Therapeutics