RT Journal Article
SR Electronic
T1 Age-Related Deficits in the Cerebellar <em>Beta</em>Adrenergic Signal Transduction Cascade in Fischer 344 Rats
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 965
OP 971
VO 281
IS 2
A1 Gould, Thomas J.
A1 Bickford, Paula C.
YR 1997
UL http://jpet.aspetjournals.org/content/281/2/965.abstract
AB Localization of age-related deficits in the cerebellarbeta adrenergic signal transduction cascade were investigated electrophysiologically using forskolin (FORSK) and adenosine-3′,5′-cyclic monophosphothioate Sp-isomer (Sp-cAMPS) appliedvia pressure ejection from extracellular multibarreled glass electrodes to activate the transduction cascade. In young rats, 100 μM FORSK activated AC, and 100 μM Sp-cAMPS activated protein kinase A; thus, both increased GABAergic inhibition of Purkinje cell firing. In aged rats, however, 100 μM FORSK was unable to increase GABAergic inhibition of Purkinje cell firing. In addition, 1 mM 7β-decacetyl-7β-(γ-N-methylpiperazino)butyryl-forskolin, an analog of FORSK, was also unable to increase GABAergic inhibition in aged rats. In contrast, Sp-cAMPS was able to increase GABAergic inhibition in aged rats, but higher doses were required than in young rats. Isoproterenol (ISO), a beta adrenergic agonist, was ineffective in increasing GABAergic inhibition of Purkinje firing in aged rats when tested alone, but ISO was effective in increasing Purkinje cell inhibition when ISO was tested with Sp-cAMPS. The results of this experiment indicate that one age-related deficit in the cerebellar beta adrenergic system occurs at the level of protein kinase A activation. The American Society for Pharmacology and Experimental Therapeutics