PT  - JOURNAL ARTICLE
AU  - G Endemann
AU  - Y Feng
AU  - C M Bryant
AU  - G S Hamilton
AU  - J Perumattam
AU  - R E Mewshaw
AU  - D Y Liu
TI  - Novel anti-inflammatory compounds prevent CD11b/CD18, alpha M beta 2 (Mac-1)-dependent neutrophil adhesion without blocking activation-induced changes in Mac-1.
DP  - 1996 Jan 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 5--12
VI  - 276
IP  - 1
4099  - http://jpet.aspetjournals.org/content/276/1/5.short
4100  - http://jpet.aspetjournals.org/content/276/1/5.full
SO  - J Pharmacol Exp Ther1996 Jan 01; 276
AB  - Leumedins are small organic molecules with anti-inflammatory properties in vivo. We report here that leumedins inhibit the CD11b/CD18 alpha M beta 2 (Mac-1)-dependent adherence of neutrophils to serum proteins. The activation of neutrophils leading to adherence via Mac-1 is associated with an increase in cell surface Mac-1 level, and with an increased affinity of Mac-1 for adhesion partners. Inhibition of neutrophil adherence by leumedins does not require blocking the recruitment of Mac-1 from intracellular granules to the cell surface. Furthermore, leumedins do not block the expression on Mac-1 of the epitope for an "activation-specific" antibody (CBRM1/5). Time course studies show that leumedins inhibit adherence by targeting an event which occurs concurrently with changes in Mac-1 level and induction of the CBRM1/5 epitope. Therefore, leumedins block an unknown process which is permissive for Mac-1-dependent adherence.