@article {Duci{\'c}438, author = {I Duci{\'c} and H J Caruncho and W J Zhu and S Vicini and E Costa}, title = {gamma-Aminobutyric acid gating of Cl- channels in recombinant GABAA receptors.}, volume = {272}, number = {1}, pages = {438--445}, year = {1995}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {We studied gamma-aminobutyric acid (GABA) gated whole-cell Cl- currents after rapid transmitter application (\< 10 ms) to cells expressing structurally different recombinant GABAA receptors. A variety of recombinant receptors were transiently transfected in a human cell line with three cDNAs encoding for different molecular forms of alpha-, beta- and gamma-subunits of the GABAA receptors. The maximal current amplitude elicited by GABA on recombinant GABAA receptors was greater when the alpha-subunits were combined with beta 2 gamma 2-subunits than with beta 1 gamma 2-subunits, with the exception of receptors including the alpha 6-subunit. The maximal current amplitude elicited by GABA was greater when the beta-subunits were combined with gamma 2 alpha 1-subunits than with gamma 1 alpha 1 subunits, with the exception of receptors including the beta 3-subunit. Furthermore, receptors comprising the beta 3-subunit had a greater sensitivity to GABA than those including the beta 1-or beta 2-subunits. When the gamma-subunits were substituted in receptors including alpha- and beta-subunits, the greatest current amplitude elicited by GABA was obtained with the alpha 1 beta 2 gamma 2 combination, whereas the GABA potency was greater with alpha 1 beta 2 gamma 1 and alpha 1 beta 1 gamma 3 than with the alpha 1 beta 2 gamma 2 combination. In receptors including alpha 6 beta 2-subunits the rank order of GABA maximal current amplitude was gamma 1 \> gamma 2 \> delta subunits.}, issn = {0022-3565}, URL = {https://jpet.aspetjournals.org/content/272/1/438}, eprint = {https://jpet.aspetjournals.org/content/272/1/438.full.pdf}, journal = {Journal of Pharmacology and Experimental Therapeutics} }