PT  - JOURNAL ARTICLE
AU  - B Meister
AU  - A Dagerlind
AU  - A P Nicholas
AU  - T Hökfelt
TI  - Patterns of messenger RNA expression for adrenergic receptor subtypes in the rat kidney.
DP  - 1994 Mar 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 1605--1611
VI  - 268
IP  - 3
4099  - http://jpet.aspetjournals.org/content/268/3/1605.short
4100  - http://jpet.aspetjournals.org/content/268/3/1605.full
SO  - J Pharmacol Exp Ther1994 Mar 01; 268
AB  - The distribution of mRNA for the rat alpha-1 A/D, alpha-1B, alpha-2A/D (RG20), alpha-2B (RNG), alpha-2C (RG10), beta-1 and beta-2 adrenergic receptors were studied in the rat kidney using in situ hybridization. After hybridized sections were exposed to autoradiography film or dipped in photographic emulsion and counterstained with hematoxylin and eosin, specific and selective labeling patterns characteristic for each probe in the kidney were observed. Labeling with the probe to the alpha-1A/D receptor was only observed in vessels in the renal parenchyma and in the ureter. Alpha-1B receptor mRNA was demonstrated in the outer and inner stripe of the outer medulla, corresponding to segment S3 of proximal tubules and the thick ascending limb of loop of Henle. Alpha-2A/D receptor mRNA was distributed in the inner stripe of the outer medulla and in the inner medulla, corresponding to collecting tubules, and in the ureter. The strongest signal in the kidney was obtained with the alpha-2B receptor probe, showing labelling in the outer stripe of the outer medulla with tubular rays radiating into the cortex, coinciding with segment S3 of proximal tubules. Weak labeling obtained with the alpha-2C receptor probe was present in the renal medulla. Labeling obtained with the probe to the beta-1 receptor was seen in the entire cortex and to a lesser extent also in the outer medulla. In addition, beta-1 receptor mRNA was shown in perirenal adipose tissue and in the ureter. Labeling obtained with the probe to the beta-2 receptor was demonstrated in the outer and inner stripe of the outer medulla.(ABSTRACT TRUNCATED AT 250 WORDS)