PT  - JOURNAL ARTICLE
AU  - R F Klein
AU  - R A Nissenson
AU  - G J Strewler
TI  - Pertussis toxin inhibits hormonal stimulation of bone resorption in fetal rat limb bones.
DP  - 1991 Sep 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 877--881
VI  - 258
IP  - 3
4099  - http://jpet.aspetjournals.org/content/258/3/877.short
4100  - http://jpet.aspetjournals.org/content/258/3/877.full
SO  - J Pharmacol Exp Ther1991 Sep 01; 258
AB  - The cellular basis for hormonal control of bone resorption is poorly understood. As the identifiable receptors for bone resorbing agents such as parathyroid hormone (PTH) and 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] are located on osteoblasts rather than osteoclasts, the nature of cellular signaling is obscure. Here it is reported that exposure of fetal rat limb bones to pertussis toxin, a bacterial protein that inhibits certain GTP binding proteins (G-proteins) involved in signal transduction, markedly inhibits bone resorption elicited by PTH, 1,25(OH)2D3 and prostaglandin E2. Pertussis toxin does not block the inhibition of alkaline phosphatase activity by PTH or 1,25(OH)2D3, and it potentiates the cyclic AMP response to PTH. These data support the existence of a pertussis toxin-sensitive G-protein that participates in regulation of bone resorption. The putative G-protein is apparently not involved in the initial transduction of hormonal signals, but it may be part of a final common pathway through which the osteoclast is activated by agents with widely divergent initial actions.