PT - JOURNAL ARTICLE AU - K Manabe AU - H Shirahase AU - H Usui AU - K Kurahashi AU - M Fujiwara TI - Endothelium-dependent contractions induced by angiotensin I and angiotensin II in canine cerebral artery. DP - 1989 Oct 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 317--320 VI - 251 IP - 1 4099 - http://jpet.aspetjournals.org/content/251/1/317.short 4100 - http://jpet.aspetjournals.org/content/251/1/317.full SO - J Pharmacol Exp Ther1989 Oct 01; 251 AB - Whether angiotensin I and angiotensin II caused endothelium-dependent contraction was examined in canine cerebral arteries. In endothelium-intact preparations, angiotensin I and angiotensin II at 10(-8) to 10(-6) M caused dose-dependent contractions, whereas both angiotensins caused much less contractions in endothelium-removed preparations. The contractions induced by angiotensin I and angiotensin II were strongly attenuated by aspirin (cyclooxygenase inhibitor) (5 x 10(-5) M), OKY-046 [thromboxane (TX) A2 synthetase inhibitor] (10(-5) M) and ONO-3708 (TX)A2 antagonist) (5 X 10(-9) M). Captopril (10(-6) M) significantly attenuated the contractions induced by angiotensin I but not those induced by angiotensin II. Angiotensin I- and angiotensin II- induced contractions were inhibited markedly by Sar1, Ala8-angiotensin II (10(-9) and 10(-8) M). The present experiments demonstrate that angiotensin I and angiotensin II produce endothelium-dependent contraction in canine cerebral artery via a factor which appears to be TXA2. Angiotensin I may be converted by endothelial cells to angiotensin II, which may activate the cells to produce TXA2 in canine cerebral artery.