PT  - JOURNAL ARTICLE
AU  - R A Cohen
TI  - Inhibition of adrenergic neurotransmission in canine tibial artery after exposure to 5-hydroxytryptamine in vitro.
DP  - 1987 Aug 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 493--499
VI  - 242
IP  - 2
4099  - http://jpet.aspetjournals.org/content/242/2/493.short
4100  - http://jpet.aspetjournals.org/content/242/2/493.full
SO  - J Pharmacol Exp Ther1987 Aug 01; 242
AB  - Blood vessels may be exposed to 5-hydroxytryptamine when platelets aggregate and release vasoactive substances at sites of damage or disease. The functional consequences were studied of exposing the dog tibial artery for 2 hr to 5-hydroxytryptamine (10(-6) M) in vitro. During the exposure, unmetabolized 5-hydroxytryptamine was accumulated by the cocaine-sensitive amine uptake mechanism of tibial artery adrenergic nerves. After exposure to [3H]-5-hydroxytryptamine, transmural electrical stimulation caused the release of the tritiated indoleamine which was blocked by tetrodotoxin. After a 1-hr washout of rings of tibial artery exposed previously to 5-hydroxytryptamine, contractions in response to transmural electrical stimulation were depressed, whereas the response to exogenously added norepinephrine was unaffected. That the decreased response to electrical stimulation after exposure to 5-hydroxytryptamine was due to decreased release of norepinephrine from adrenergic nerves was demonstrated in strips of the artery preincubated in [3H]norepinephrine. The inhibition of [3H]norepinephrine release after exposure to 5-hydroxytryptamine, was blocked by the serotonergic antagonist, methiothepin, but not by the alpha adrenergic antagonist, phentolamine, suggesting that serotonergic receptors mediate the inhibition. The inhibition of [3H]norepinephrine release also was prevented by blocking adrenergic neuronal uptake with cocaine before exposure to 5-hydroxytryptamine. These results suggest that 5-hydroxytryptamine is accumulated and released by tibial artery adrenergic nerves as a cotransmitter. In so doing, the indoleamine inhibits adrenergic neurotransmission in the tibial artery by its action at prejunctional serotonergic receptors.