RT Journal Article
SR Electronic
T1 Glutathione biosynthesis from sulfur-containing amino acids in enriched populations of Clara and type II cells and macrophages freshly isolated from rabbit lung.
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 376
OP 380
VO 240
IS 2
A1 J K Horton
A1 M J Meredith
A1 J R Bend
YR 1987
UL http://jpet.aspetjournals.org/content/240/2/376.abstract
AB The glutathione content of freshly isolated rabbit alveolar type II cells, Clara cells and macrophages was depleted by incubating with diethyl maleate and the ability of these cell types to resynthesize glutathione from the sulfur-containing amino acids, cysteine, cystine and methionine was determined. Cysteine and methionine were taken up at a similar rate into each of the cell types, with cystine uptake occurring at a 10- to 14-fold lower rate. The cells were best able to utilize cysteine for glutathione resynthesis and the rates of both uptake and synthesis were fastest in macrophages and slowest in type II cells. There was no evidence for participation of a cystathionine pathway for glutathione synthesis in any of the cell types.