PT  - JOURNAL ARTICLE
AU  - P C Ferriola
AU  - M A Acara
AU  - M E Duffey
TI  - Thiazide diuretics inhibit chloride absorption by rabbit distal colon.
DP  - 1986 Sep 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 912--915
VI  - 238
IP  - 3
4099  - http://jpet.aspetjournals.org/content/238/3/912.short
4100  - http://jpet.aspetjournals.org/content/238/3/912.full
SO  - J Pharmacol Exp Ther1986 Sep 01; 238
AB  - In order to investigate the cellular mechanisms of action of thiazide diuretics, the effect of diuretic and nondiuretic thiazide compounds on Cl- absorption across rabbit distal colon was assessed in tissues mounted in Ussing chambers. This epithelium absorbs Cl- via an active electroneutral transport process. Net 36Cl- absorption across short-circuited tissues was decreased 53, 36 and 20% after addition of 10(-4) M trichlormethiazide, bendroflumethiazide or hydrochlorothiazide, respectively, to the mucosal bathing solution. This inhibitory effect was a result of a decrease in the mucosa-to-serosa unidirectional Cl- flux (P less than .02). Neither the serosa-to-mucosa Cl- flux nor Isc was affected by the thiazides. Thiazide diuretics may exert their effect on Cl- transport across rabbit distal colon through inhibition of a Cl(-)-HCO-3 exchange mechanism. The nondiuretic thiazide, diazoxide, had no effect on Cl- transport. The similarity between the diuretic potency of these compounds and their potency as inhibitors of Cl- absorption by rabbit colon suggests that the thiazides have a similar mechanism of action in renal epithelia.