PT - JOURNAL ARTICLE AU - J H Bowdre AU - J H Hull AU - D M Cocchetto TI - Antibiotic efficacy against Vibrio vulnificus in the mouse: superiority of tetracycline. DP - 1983 Jun 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 595--598 VI - 225 IP - 3 4099 - http://jpet.aspetjournals.org/content/225/3/595.short 4100 - http://jpet.aspetjournals.org/content/225/3/595.full SO - J Pharmacol Exp Ther1983 Jun 01; 225 AB - Seven antimicrobial agents, all effective against Vibrio vulnificus in vitro, were compared for in vivo efficacy in mice experimentally infected with V. vulnificus strain B3547. Mice were injected s.c. with 1 X 10(8) cells, and i.p. injection of antimicrobials was begun 1.5 hr later when mice were bacteremic and had edematous lesions at the injection site. The study was done in two phases. Phase I was a dose-ranging experiment, using single injections within the range (on a body weight-adjusted basis) clinically useful in humans. Of 12 mice treated with tetracycline (4 mg/kg), 12 survived at 24 hr, compared to 0 of 21 saline-treated controls, 3 of 10 given ampicillin (32 mg/kg) and 2 of 3 given cefotaxime (20 mg/kg). There were no survivors at 24 hr in groups of 5 to 10 mice treated with cefazolin (32 mg/kg), carbenicillin (80 mg/kg), erythromycin (8 mg/kg) or gentamicin (8 mg/kg). Phase II was designed to simulate clinical conditions using multiple dosing for 30 hr and scoring for survival at 96 hr. Of 12 mice given tetracycline (3 mg/kg every 12 hr), 12 survived, compared to 1 of 10 given cefotaxime (20 mg/kg every 6 hr), 0 of 12 given carbenicillin (40 mg/kg every 6 hr) and 0 of 12 given saline every 6 hr. Tetracycline thus appears to be the agent of choice among those tested for in vivo efficacy against V. vulnificus in the mouse model.