PT  - JOURNAL ARTICLE
AU  - N Ackerman
AU  - A Tomolonis
AU  - L Miram
AU  - J Kheifets
AU  - S Martinez
AU  - A Carter
TI  - Three day pleural inflammation: a new model to detect drug effects on macrophage accumulation.
DP  - 1980 Dec 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 588--595
VI  - 215
IP  - 3
4099  - http://jpet.aspetjournals.org/content/215/3/588.short
4100  - http://jpet.aspetjournals.org/content/215/3/588.full
SO  - J Pharmacol Exp Ther1980 Dec 01; 215
AB  - Mononuclear cell accumulation is of major importance in maintaining chronic inflammatory conditions. In an effort to model this phenomenon, 0.3 ml of a 1% carrageenan solution was injected into the pleural cavity of rats; at various times thereafter peripheral blood and pleural exudate samples were collected. Seventy-two hours after carrageenan injection, 82.3 +/- 3.7 x 10(6) cells (N = 6; mean +/- S.E.) were present in the pleural cavity; over 80% of these cells were macrophages as determined by morphologic and histochemical criteria. Animals treated with dexamethasone had a significantly reduced number of pleural macrophages. Animals treated with the nonsteroidal anti-inflammatory agents, naproxen and indomethacin, had an elevated intrapleural macrophage content. The number of intrapleural cells was not affected by the antirheumatic agents levamisole, d- and dl-penicillamine or gold sodium thiomalate. Animals treated with tilorone, dapsone, hydroxychloroquine, phenylmethane-sulfonyl fluoride and 1,10 phenanthroline had a reduced pleural cell count.