PT  - JOURNAL ARTICLE
AU  - P Dutta
AU  - S J Mustafa
TI  - Binding of adenosine to the crude plasma membrane fraction isolated from dog coronary and carotid arteries.
DP  - 1980 Sep 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 496--502
VI  - 214
IP  - 3
4099  - http://jpet.aspetjournals.org/content/214/3/496.short
4100  - http://jpet.aspetjournals.org/content/214/3/496.full
SO  - J Pharmacol Exp Ther1980 Sep 01; 214
AB  - In an attempt to elucidate the mechanism of action of adenosine, specific adenosine binding was determined by using plasma membrane rich microsomal fraction of dog coronary and carotid arteries. The binding of adenosine to both coronary and carotid preparations was temperature as well as pH dependent and was inhibited by aminophylline and adenosine nucleotides. Scatchard analysis of the saturable binding data on carotid arteries exhibited a single species of binding sites with a Kd value of 1.34 x 10(-6) M and binding capacity of 140 pmol/mg of protein. The first order dissociation rate constant of adenosine binding to carotid preparation was 0.0247 min-1. The nonlinearity of equilibrum binding data (Scatched Plot) and dissociation curve of binding to coronary arteries suggested the presence of more than one population of binding sites. The data suggest that the binding sites located in the microsomal fraction of coronary and carotid arteries possess the basic characteristics of the receptors which might be involved in the vasodilatory action of adenosine.