PT  - JOURNAL ARTICLE
AU  - K W Renton
TI  - The deleterious effect of Bordetella pertussis vaccine and poly(rI.rC) on the metabolism and disposition of phenytoin.
DP  - 1979 Feb 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 267--270
VI  - 208
IP  - 2
4099  - http://jpet.aspetjournals.org/content/208/2/267.short
4100  - http://jpet.aspetjournals.org/content/208/2/267.full
SO  - J Pharmacol Exp Ther1979 Feb 01; 208
AB  - The elimination of phenytoin from blood was significantly decreased in rats pretreated 24 hr previously with Bordetella pertussis vaccine or the synthetic polynucleotide poly(rI.rC.). Phenytoin half-life was increased about 4-fold by both agents. In microsomes prepared from rats pretreated with B. pertussis vaccine or poly(rI.rC) the hydroxylation of phenytoin in vitro was decreased by 36 nad 53%, respectively. The addition of these agents to normal microsomal suspensions had no effect on phenytoin hydroxylation, demonstrating that the decrease in biotransformation was not due to direct enzyme inhibition. The decrease in phenytoin hydroxylation and elimination rate resulted from depressed levels of cytochrome P-450 in the hepatic endoplasmic reticulum of rats treated with B. pertussis vaccine or poly(rI.rC).