@article {PERKINS371, author = {JOHN P. PERKINS and MARILYN M. MOORE}, title = {CHARACTERIZATION OF THE ADRENERGIC RECEPTORS MEDIATING A RISE IN CYCLIC 3{\textquoteright},5{\textquoteright}-ADENOSINE MONOPHOSPHATE IN RAT CEREBRAL CORTEX}, volume = {185}, number = {2}, pages = {371--378}, year = {1973}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Norepinephrine (NE) causes a 4- to 6-fold rise in the cyclic 3{\textquoteright}, 5{\textquoteright}-adenosine monophosphate content of chopped rat cerebral cortex. The effect is half-maximal at 6 {\textmu}M and essentially maximal at 30 {\textmu}M. The effect of 30 {\textmu}M NE is only partially blocked by maximally effective concentrations (30 {\textmu}M) of either propranolol or phentolamine. However, the effect is completely blocked when these inhibitors are added together even at lower concentrations (10 {\textmu}M each). Isoproterenol (ISO) causes only a 2- to 4-fold rise in cyclic 3{\textquoteright}, 5{\textquoteright}-adenosine monophosphate in the same preparation. The effect is half-maximal at 0.4 {\textmu}M and essentially maximal at 3 {\textmu}M. The effect of 30 {\textmu}M ISO is completely blocked by 30 {\textmu}M propranolol, but phentolamine is without effect. Maximally effective concentrations of NE and ISO together produce an effect similar to that of NE added alone. The results suggest that NE interacts with at least two types of receptors (analogous to alpha and beta) whereas ISO appears to react with a single type (analagous to beta). Analysis of the difference in the time courses of the effects of NE and ISO suggests that a slow component of the NE effect may be indirect. {\textcopyright} 1973 by The Williams \& Wilkins Co.}, issn = {0022-3565}, URL = {https://jpet.aspetjournals.org/content/185/2/371}, eprint = {https://jpet.aspetjournals.org/content/185/2/371.full.pdf}, journal = {Journal of Pharmacology and Experimental Therapeutics} }