PT  - JOURNAL ARTICLE
AU  - E. X. ALBUQUERQUE
AU  - J. E. WARNICK
AU  - F. M. SANSONE
AU  - J. DALY
TI  - THE PHARMACOLOGY OF BATRACHOTOXIN. V. A COMPARATIVE STUDY OF MEMBRANE PROPERTIES AND THE EFFECT OF BATRACHOTOXIN ON SARTORIUS MUSCLES OF THE FROGS <em>PHYLLOBATES AUROTAENIA</em> AND <em>RANA PIPIENS</em>
DP  - 1973 Feb 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 315--329
VI  - 184
IP  - 2
4099  - http://jpet.aspetjournals.org/content/184/2/315.short
4100  - http://jpet.aspetjournals.org/content/184/2/315.full
SO  - J Pharmacol Exp Ther1973 Feb 01; 184
AB  - Membrane properties and the effects of batrachotoxin (BTX) and tetrodotoxin were studied on sartorius muscles of Rana pipiens and of Phyllobates aurotaenia, the frog which produces BTX. There was no significant difference in the resting membrane potential, amplitude and time course of miniature end-plate potentials or the quantum content recorded in sartorius muscles of the two frogs. The frequency of spontaneous transmitterrelease was, however, higher in the sartorius muscles of P. aurotaenia than in R. pipiens. Both the time constant and specific resistance of a unit area of membrane of the sartorius muscles of P. aurotaenia were significantly lower (P < .01) than those of R. pipiens muscle fibers. The intracellularly recorded action potential of sartorius muscles from both frogs was blocked by removal of the external sodium or by exposure to tetrodotoxin. BTX (1.2 x 10-6 M) had no effect on membrane potential, miniature end-plate potential amplitude and frequency, spike generation or twitch of the nerve-muscle preparations of P. aurolaenia. When sartorius muscles of R. pipiens were stimulated at a frequency of 0.2 puLse/sec and exposed to BTX (4.0 x 10-8 M), the indirectly elicited muscle twitch was blocked and all surface fibers were depolarized within 13 minutes: simultaneously, there was a transient muscle contracture lasting seven to eight minutes. Veratridine (7.5 x 10-6 M) depolarized the surface fibers of stimulated sartorius muscles from P. anrotaenia by 20 mV after one hour but those of R. pipiens were depolarized by 95 mV within 15 minutes. BTX-depolarized sartorius muscles of R. pipiens were insensitive to isotonic KCl, whereas caffeine (20 mM) induced a normal contracture. Electron microscopic obesrvations of BTX-treated sartorius muscles from R. pipiens revealed damage to only the presynaptic © 1973 by The Williams & Wilkins Co.