RT Journal Article
SR Electronic
T1 INDUCTION OF DOPAMINE-β-HYDROXYLASE AND TYROSINE HYDROXYLASE IN RAT HEARTS AND SYMPATHETIC GANGLIA
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 116
OP 129
VO 182
IS 1
A1 MOLINOFF, PERRY B.
A1 BRIMIJOIN, STEPHEN
A1 AXELROD, JULIUS
YR 1972
UL http://jpet.aspetjournals.org/content/182/1/116.abstract
AB The administration of reserpine caused an increase in the activity of dopamine-β-hydroxylase in rat hearts and stellate ganglia and of tyrosine hydroxylase (measured in ganglia only). The effect of reserpine on dopamine-β-hydroxylase was dose-dependent, was reduced by the ganglionic blocking agent, chlorisondamine and was prevented by the inhibitor of protein synthesis, cycloheximide. The effect was not prevented by the simultaneous administration of the inhibitor of messenger ribonucleic acid synthesis, actinomycin D. The Km of dopamine-β-hydroxylase for phenylethylamine in stellate ganglia was not affected by reserpine pretreatment sufficient to cause a 2½-fold increase in the Vmax. The turnover of dopamine-β-hydroxylase, measured as the rate of decline in enzyme activity after inhibition of protein synthesis, was the same in stellate ganglia from reserpine-treated and control rats. The increase in the activity of both tyrosine hydroxylase and dopamine-β-hydroxylase was partially or completely prevented by the administration of dopa, bretylium or inhibitors of monoamine oxidase. Depletion of catecholamines by the inhibitor of tyrosine hydroxylase, α-methyl-p-tyrosine, also led to an increase in dopamine-β-hydroxylase activity. The administration of dopa or dopamine did not change the activity of dopamine-β-hydroxylase in ganglia but did cause a decrease in its activity in the heart. © 1972, by The Williams & Wilkins Company