RT Journal Article
SR Electronic
T1 EFFECT OF RESERPINE, PHENOXYBENZAMINE AND COCAINE ON NEUROMUSCULAR TRANSMISSION IN THE VAS DEFERENS OF THE GUINEA PIG
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 310
OP 317
VO 181
IS 2
A1 ARUN R. WAKADE
A1 JOHN KRUSZ
YR 1972
UL http://jpet.aspetjournals.org/content/181/2/310.abstract
AB Chemical analysis of vasa deferentia taken from guinea pigs pretreated with high doses of reserpine showed almost complete depletion of their norepinephrine stores. However, the contractile response of the reserpine-pretreated vas deferens to hypogastric nerve stimulation was still maintained at 60% of the control level. In vitro treatment with reserpine (1.6 x 10-7 to 4.1 x 10-5 M) had no inhibitory effect on neuromuscular transmission in the vas deferens. Phenoxybenzamine was found to be ineffective in blocking nerve-mediated responses of the vas deferens obtained from reserpine-pretreated guinea pigs. In fact, phenoxybenzamine, (1.77-2.95 x 10-4 M) markly potentiated (about 5-fold) nervemediated responses and, in addition, always induced spontaneous contractions of the reserpine-pretreated vas deferens. In the presence of cocaine (3.2 x 10-6 to 9.8 x 10-5 M), contractions of the reserpine-pretreated vas deferens induced by hypogastric nerve stimulation remained totally unaffected, whereas responses to exogenous norepinephrine were augmented nearly 300-fold. © 1972 by The Williams & Wilkins Co.