PT  - JOURNAL ARTICLE
AU  - PENDLETON, ROBERT G.
AU  - NEWMAN, DAVID J.
AU  - SHERMAN, SHELDON S.
AU  - BRANN, EDWARD G.
AU  - MAYA, WILLIAM E.
TI  - EFFECT OF PROPRANOLOL UPON EFFECT THE HEMOGLOBIN-OXYGEN DISSOCIATION CURVE
DP  - 1972 Mar 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 647--656
VI  - 180
IP  - 3
4099  - http://jpet.aspetjournals.org/content/180/3/647.short
4100  - http://jpet.aspetjournals.org/content/180/3/647.full
SO  - J Pharmacol Exp Ther1972 Mar 01; 180
AB  - Propranolol (1-5 x 10-4 M) was found to shift the hemoglobin-oxygen dissociation curve of human erythrocytes significantly to the right; the mean p50 was increased in various studies by 6 to 8 mm Hg. The dissociation curve was measured in vitro on freshly obtained, washed red blood cells previously incubated for 90 minutes in physiological buffer (pH 7.4, T = 37°C) which contained the test compound. Concomitant with shifting the curve, propranolol also altered the morphology of the red blood cells and reduced the hematocrit. d-Propranolol was more effective than the l-form in shifting the curve and INPEA, MJ 1999 and Kö 592 were ineffective at 5 x 10-4 M, indicating the effect was not beta receptor mediated. Propranolol did not affect the dissociation curve of dialyzed hemoglobin or of erythrocytes obtained from out-dated blood. Biochemically, the drug significantly decreased the erythrocyte content of 2,3-diphosphoglyceric acid and adenosine triphosphate and inhibited glucose utilization by ∼60%. The onset of propranolol action on cellular morphology and the dissociation curve was essentially instantaneous and peaked within approximately 20 minutes which suggests these effects may be membrane mediated. © 1972 by The Williams & Wilkins Co.