TY - JOUR T1 - EFFECT OF CARBON TETRACHLORIDE-INDUCED LIVER DAMAGE ON HEPATIC TRANSPORT OF OUABAIN IN THE RAT JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 589 LP - 594 VL - 178 IS - 3 AU - RICHARD H. REUNING AU - LEWIS S. SCHANKER Y1 - 1971/09/01 UR - http://jpet.aspetjournals.org/content/178/3/589.abstract N2 - The effect of carbon tetrachloride (CCL4) pretreatment on the hepatic transport of ouabain was investigated in anesthetized rats with ligated renal pedicles and a cannulated bile duct. Each animal received a single i.p. injection of CCL4 (1 ml/kg) as a 50% solution in olive oil at times ranging from 0.5 to 71.5 hours prior to an i.v. injection of ouabain-3H, 1 mg/kg. Control animals were pretreated with olive oil alone prior to the ouabain injection. The concentration of ouabain in liver and plasma was much greater in CCl4-treated rats than in controls when measured 30 minutes after injection of the glycoside. In contrast, the rate of biliary excretion of ouabain during the 30-minute period was much lower in CCl4-treated rats than in controls. These effects were seen at 1, 4, 24 and 72 hours after CCl4 pretreatment and were most pronounced after 24 hours. In both treated and control rats, ouabain appeared in bile only as the unchanged compound and was not appreciably bound to liver or plasma proteins. Analysis of the data indicates that the observed effects of CCl4 treatment are due to a marked depression of transport of ouabain from liver to bile. © 1971 by The Williams & Wilkins Co. ER -