RT Journal Article
SR Electronic
T1 SELECTIVE RELEASE OF HISTAMINE FROM RAT MAST CELLS BY SEVERAL DRUGS
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 627
OP 631
VO 175
IS 3
A1 HARRY V. ELLIS III
A1 ALICE R. JOHNSON
A1 NEIL C. MORAN
YR 1970
UL http://jpet.aspetjournals.org/content/175/3/627.abstract
AB Polymyxin B, d-tubocurarine and morphine released granule-bound histamine from rat mast cells, in vitro, but not the cytoplasmic enzyme lactic dehydrogenase. Rat mast cells treated with any of these drugs or with stilbamidine were not stained by the vital dye trypan blue. because of the fluorescence of stilbamidine, release of lactic acid dehydrogenase could not be measured in the presence of this drug. It is concluded that these four drugs release histamine by the "selective" degranulation mechanism described for compound 48/80, rather than the "nonselective" mechanism described for Triton X-100, which is characterized by destruction of cell membranes, resulting in release of cytoplasmic constituents and uptake of trypan blue. The order of potency as histamine releasers in 48/80 ≥ polymyxin B > stilbamidine > d-tubocurarine > morphine. All basic, small-molecule histamine releasers, except the aliphatic amines, may be selective releasers. Some of the clinically observed side effects of these drugs are probaby due to histamine release. © 1970 by The Williams & Wilkins Co.