PT  - JOURNAL ARTICLE
AU  - BERNDT, W. O.
AU  - GROTE, DACE
TI  - THE ACCUMULATION OF C<sup>14</sup>-DINITROPHENOL BY SLICES OF RABBIT KIDNEY CORTEX
DP  - 1968 Nov 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 223--231
VI  - 164
IP  - 1
4099  - http://jpet.aspetjournals.org/content/164/1/223.short
4100  - http://jpet.aspetjournals.org/content/164/1/223.full
SO  - J Pharmacol Exp Ther1968 Nov 01; 164
AB  - Standard tissue-slice techniques have been employed to examine the uptake of the metabolic inhibitor, 2, 4-dinitrophenol (DNP) by rabbit renal cortex. For this study the uptake of C14-dinitrophenol (C14-DNP) was monitored. No metabolism of the C14-DNP by slices of renal cortex was detected with paper chromatography. Steady-state slice/medium ratios of 10 to 15 were attained in 40 to 60 mm. Various metabolic inhibitors (e.g., sodium cyanide, iodoacetamide, p-chloromercuribenzoate) reduced the accumulation significantly. Although some metabolic intermediates (e.g., acetate, glucose) failed to influence the C14 accumulation, most reduced the uptake. Other organic acids known to be transported by the renal organic anion transport system also inhibited the C14-DNP uptake (e.g., octanoate, decanoate, p-aminohippurate). Various organic bases were tested but did not influence DNP accumulation. Furthermore, alterations in the potassium ion concentration of the bathing solution did not affect DNP uptake. Also, no pH optimum was found. It was concluded that the accumulation of C14-DNP occurred by two processes: 1) active uptake by the renal organic acid transport process, and 2) a nonspecific binding or partitioning. © 1968, by The Williams & Wilkins Company