RT Journal Article SR Electronic T1 THE REVERSIBLE AND IRREVERSIBLE INHIBITION OF N1-METHYLNICOTINAMIDE UPTAKE INTO RAT KIDNEY CORTEX SLICES JF Journal of Pharmacology and Experimental Therapeutics JO J Pharmacol Exp Ther FD American Society for Pharmacology and Experimental Therapeutics SP 461 OP 467 VO 163 IS 2 A1 ALAN M. REYNARD YR 1968 UL http://jpet.aspetjournals.org/content/163/2/461.abstract AB The uptake of N1-methyinicotinamide (NMN) and p-aminohippuric acid by rat kidney cortex slices was measured. Measurement of inhibition of NMN uptake by various NMN analogs has shown that inhibition is not favored at any particular position on the ring. Inhibition by a series of N1-alkylnicotinamides demonstrated an increasing interaction between inhibitor and transport system as the alkyl chain was increased from two to six carbons with no further increase in interaction past six carbons. The partition of the N1-alkylnicotinamides between 1-octanol and water is reported, and the possible hydrophobic interaction between these compounds and the transport system is discussed. The conclusions based on the N1- alkylnicotinamide experiments are substantiated by use of a series of irreversible inbibitions, 2-haloethylamines. The use of the information in the design of further irreversible inhibitors and the application of these inhibitors in the isolation of the membrane proteins involved in uptake of NMN are discussed. © 1968 by the Williams & Wilkins Co.