PT  - JOURNAL ARTICLE
AU  - JULIUS C. ALLEN
AU  - ARNOLD SCHWARTZ
TI  - A POSSIBLE BIOCHEMICAL EXPLANATION FOR THE INSENSITIVITY OF THE RAT TO CARDIAC GLYCOSIDFJS
DP  - 1969 Jul 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 42--46
VI  - 168
IP  - 1
4099  - http://jpet.aspetjournals.org/content/168/1/42.short
4100  - http://jpet.aspetjournals.org/content/168/1/42.full
SO  - J Pharmacol Exp Ther1969 Jul 01; 168
AB  - The inhibitory effect of ouabain on Na+, K+-adenosine triphosphatase (Na+, K+-ATPase) from rat kidney and heart was found to be both temperature-and time-insensitive. Stoichiometric studies of H3-glycoside binding to rat enzyme preparations revealed a 2:1 or 3:1 relationship, whereas beef and dog preparations exhibited a 1:1 relationship (amount of glycoside bound per unit of enzyme activity). The beef and dog Na+, K+-ATPases were significantly more sensitive than rat Na+, K+-ATPases to both ouabain-induced inhibition and H3-glycoside binding. The complex formed between cardiac glycoside and the Na+ , K+-ATPase of digitalis-sensitive species appears to be much more stable than the complex formed between the Na+, K+-ATPase of rat and the glycoside. The data suggest that specific characteristics of the rat Na+, K+-ATPase which differ from those of sensitive species may explain the insensitivity of the rat to digitalis drugs. © 1969, by The Williams & Wilkins Company