RT Journal Article
SR Electronic
T1 METABOLISM OF DRUGS AND CARCINOGENS BY HUMAN LIVER ENZYMES
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 151
OP 156
VO 152
IS 1
A1 R. Kuntzman
A1 L. C. Mark
A1 L. Brand
A1 M. Jacobson
A1 W. Levin
A1 A. H. Conney
YR 1966
UL http://jpet.aspetjournals.org/content/152/1/151.abstract
AB Human liver contains enzyme systems which catalyze the ring hydroxylation of 3,4-benzpyrene, the side chain oxidation of pentobarbital, the O-dealkylation of acetophenetidin and the N-dealkylation of 3-methyl-4-monomethylaminoazobenzene. The rate of metabolism of these substrates by hepatic enzymes was compared in man and rat. The enzyme system which hydroxylates 3,4-benzpyrene was studied in detail. This enzyme system in man is similar to that found in the rat; both are localized in the microsomal fraction and require reduced triphosphopyridine nucleotide (NADPH) for activity. Methods are described for measuring oxidative microsomal enzyme activity in small samples of human liver. The Williams & Wilkins Comapny