PT  - JOURNAL ARTICLE
AU  - Julius Axelrod
AU  - Joseph K. Inscoe
TI  - THE UPTAKE AND BINDING OF CIRCULATING SEROTONIN AND THE EFFECT OF DRUGS
DP  - 1963 Aug 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 161--165
VI  - 141
IP  - 2
4099  - http://jpet.aspetjournals.org/content/141/2/161.short
4100  - http://jpet.aspetjournals.org/content/141/2/161.full
SO  - J Pharmacol Exp Ther1963 Aug 01; 141
AB  - A specific method for the estimation of C14-serotonin is described. The fate of intravenously administered C14-serotonin was investigated in mice and rats. In the whole mouse about half of the circulating serotonin is rapidly bound in tissues and slowly released over many days. It is proposed that binding might serve as a major mechanism for the inactivation of circulating serotonin. C14-serotonin is unequally taken up in various tissues examined. It is selectively taken up and retained by the lung, spleen and adrenal gland. Tryptamine, reserpine and imipramine increase the rate of destruction of circulating serotonin but chlorpromazine, tyramine, amphetamine, cocaine, LSD, and guanethidine have no effect. The monoamine oxidase inhibitor JB 516 slows the disappearance of serotonin. Tryptamine, reserpine, cocaine, imipramine and LSD block the uptake of serotonin in lung and spleen while chlorpromazine had no significant effect.