PT  - JOURNAL ARTICLE
AU  - James L. Way
TI  - THE METABOLISM OF C<sup>14</sup> 2-PAM IN THE ISOLATED PERFUSED RAT LIVER
DP  - 1962 Nov 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 258--263
VI  - 138
IP  - 2
4099  - http://jpet.aspetjournals.org/content/138/2/258.short
4100  - http://jpet.aspetjournals.org/content/138/2/258.full
SO  - J Pharmacol Exp Ther1962 Nov 01; 138
AB  - An ultraviolet spectrophotometric method was developed in this laboratory to follow the metabolism of 1-methyl-2-formylpyridinium iodide oxime (2-PAM) in liver perfusates. This determination was based on the bathochromic shift of 2-PAM in alkaline solutions. Approximately 0.01 µmole per ml of 2-PAM can be determined by this method. Formation of the zwitterionic species of 2-PAM was established as the form of 2-PAM which produced the bathochromic displacement. Carbon-14 labeled 2-PAM was prepared and its biotransformation was also investigated in the isolated perfused rat liver. Both ultraviolet absorption analyses and isotopic tracer studies indicated that extensive biotransformation of 2-PAM had occurred.