TY - JOUR T1 - STUDIES ON A METHOD FOR ASSAYING THE METABOLIC EFFECTS OF INSULIN ON ADIPOSE TISSUE <em>IN VITRO</em> AND <em>IN VIVO</em> JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 205 LP - 208 VL - 136 IS - 2 AU - Robert E Bagdon AU - F. Hall Y1 - 1962/05/01 UR - http://jpet.aspetjournals.org/content/136/2/205.abstract N2 - The addition of insulin in vitro to rat epididymal adipose tissue was found to stimulate the production of carbon dioxide. Maximal rates of carbon dioxide evolution were evoked by addition of final concentrations of 0.1 to 0.001 unit per ml of insulin and the threshold concentration was determined as about 0.00025 unit per ml. It was also possible to determine the influence of insulin on the metabolic activity of adipose tissue in vivo. Insulin was administered to intact rats and the baseline carbon dioxide production of the excised epididymal fat pads prior to direct addition of insulin to the adipose tissue served as a measure of the in vivo activity of the hormone. The effect of varying the dose of insulin and the time interval of sacrifice following administration of insulin on the baseline metabolic activity of adipose tissue was investigated in detail. The increase in carbon dioxide evolution caused by administration of insulin to intact rats coincided with the occurrence of hypoglycemia and could be augmented by further additions of the hormone directly to adipose tissue. The results of this investigation indicate that the manometric measurement of the carbon dioxide production by adipose tissue can serve as the basis for a sensitive assay for insulin-like activity in vitro and in vivo. ER -